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Deproteinization of primary enamel with sodium hypochlorite before phosphoric acid etching
López Luján, Nieves A; Munayco Pantoja, Evelyn R; Torres Ramos, Gilmer; Blanco Victoria, Daniel J; Siccha Macassi, Ana; López Ramos, Roxana P.
  • López Luján, Nieves A; National Major San Marcos University. School of Dentistry. Department of Pediatric Stomatology and Orthodontics. Lima. AR
  • Munayco Pantoja, Evelyn R; National Major San Marcos University. School of Dentistry. Department of Pediatric Stomatology and Orthodontics. Lima. PE
  • Torres Ramos, Gilmer; National Major San Marcos University. School of Dentistry. Department of Pediatric Stomatology and Orthodontics. Lima. PE
  • Blanco Victoria, Daniel J; National Major San Marcos University. School of Dentistry. Department of Pediatric Stomatology and Orthodontics. Lima. PE
  • Siccha Macassi, Ana; National University of the Callao. School of Health Sciences. Department of General and Organic Chemistry. Lima. PE
  • López Ramos, Roxana P; Peruvian Cayetano Heredia University. School of Public Health and Management. Postgraduate Direction Office. Lima. PE
Acta odontol. latinoam ; 32(1): 29-35, 2019. ilus, tab, graf
Article in English | LILACS | ID: biblio-1010419
ABSTRACT
The aim of this study was to evaluate the deproteinization of primary enamel by analyzing etching pattern types, with and without the application of 5% NaOCl before acid etching with 37% H3PO4. Fifteen extracted human primary molars were randomly selected for the present in vitro study; 1mm x 1mm blocks were prepared and divided into two groups (n = 21). These groups were treated as follows Group AAcid Etching with 37% H3PO4 gel for 15 s; Group B5% NaOCl for 60 s + Acid Etching with 37% H3PO4for 15 s. The specimens were prepared for scanning electron microscopy analysis. The images were evaluated for quality types I and II etching of the enamel surface using ImageJ software. Datasets were checked for normality by KolgomorvSmirnov test and the nonparametric unpaired MannWhitney test was applied. The mean surface area of type I and II etching pattern values was 1922.314 µm2for Group A and 3840.473 µm2Group B. We conclude that deproteinization with 5% NaOCl prior to acid etching can be used to increase the area of adhesion and the quality of the etching pattern (AU)
RESUMEN
El objetivo del estudio fue evaluar la desproteinización del esmalte primario a través de los tipos de patrones de grabado, con y sin NaOCl 5% utilizado antes del grabado ácido con H3PO4 37%. Quince dientes primarios humanos extraídos se seleccionaron al azar para el presente estudio in vitro, se prepararon bloques de 1mm x 1 mm y se dividieron en dos grupos (n = 21). Estos grupos se trataron de la siguiente manera Grupo A Grabado ácido con H3PO4 37% en gel durante 15 segundos; Grupo B NaOCl 5% durante 60 segundos + Grabado ácido con H3PO4 37% durante 15 segundos. Las muestras se prepararon para el análisis de microscopía electrónica de barrido. Las imágenes obtenidas se evaluaron principalmente por la calidad de los grabados tipo I y II de la superficie del esmalte primario, utilizando el software Image J. Los datos se analizaron en cuanto a su normalidad mediante la prueba de KolgomorvSmirnov, se utilizó pruebas no paramétricas Prueba de MannWhitney no pareada. Como resultado, se encontró que el área de superficie media de los valores de patrón de grabado de tipo I y II para el Grupo A era 1922,314 µm2 y el Grupo B era 3840,473 µm2. Finalmente, llegamos a la conclusión de que se puede usar la desproteinización con NaOCl 5% antes del grabado ácido para aumentar el área de adhesión y la calidad del patrón de grabado (AU)
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Phosphoric Acids / Sodium Hypochlorite / Tooth, Deciduous / Acid Etching, Dental / Dental Enamel Type of study: Evaluation studies Limits: Humans Country/Region as subject: South America / Peru Language: English Journal: Acta odontol. latinoam Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Argentina / Peru Institution/Affiliation country: National Major San Marcos University/AR / National Major San Marcos University/PE / National University of the Callao/PE / Peruvian Cayetano Heredia University/PE

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Full text: Available Index: LILACS (Americas) Main subject: Phosphoric Acids / Sodium Hypochlorite / Tooth, Deciduous / Acid Etching, Dental / Dental Enamel Type of study: Evaluation studies Limits: Humans Country/Region as subject: South America / Peru Language: English Journal: Acta odontol. latinoam Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Argentina / Peru Institution/Affiliation country: National Major San Marcos University/AR / National Major San Marcos University/PE / National University of the Callao/PE / Peruvian Cayetano Heredia University/PE