The application of Compont gel in chronic obstructive jaundice rats model
Acta cir. bras
;
34(5): e201900504, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1010871
ABSTRACT
Abstract Purpose:
To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis.Methods:
90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology.Results:
Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group.Conclusion:
Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bile Ducts
/
Disease Models, Animal
/
Gels
/
Liver Cirrhosis
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Guangxi Medical University/CN
/
Guilin Medical University/CN
/
Guilin NO. 924 Hospital/CN
/
Hebei Medical University/CN
/
Wuhan University/CN
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