MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
Biol. Res
;
51: 50, 2018. graf
Article
in English
| LILACS
| ID: biblio-1011394
ABSTRACT
BACKGROUND:
Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer.METHODS:
The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation.RESULTS:
Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3'-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo.CONCLUSION:
Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ovarian Neoplasms
/
Gene Expression Regulation, Neoplastic
/
HMGA2 Protein
/
MicroRNAs
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2018
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Tongji University School of Medicine/CN
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