Vitexin induces apoptosis through mitochondrial pathway and PI3K/Akt/mTOR signaling in human non-small cell lung cancer A549 cells
Biol. Res
;
52: 7, 2019. graf
Article
in English
| LILACS
| ID: biblio-1011410
ABSTRACT
BACKGROUND:
Currently, the prognosis of patients with non-small cell lung cancer (NSCLC) remains dismal; hence, it is critical to identify effective anti-NSCLC agents with limited side effects. This study aimed to evaluate the therapeutic potential of flavonoid compound vitexin in human NSCLC cells and the underlying mechanisms.RESULTS:
The experimental results indicated that vitexin reduced the viability of A549 cells in a dose-dependent manner with nearly no toxicity against normal human bronchial epithelial 16HBE cells. Vitexin also dose-dependently increased A549 cell apoptosis, accompanied by the decreased Bcl-2/Bax ratio and the increased expression of cleaved caspase-3. Moreover, the in vivo anticancer activity of vitexin was further determined in nude mice bearing A549 cells. In addition, vitexin induced the release of cytochrome c from the mitochondria to the cytosol and the loss of mitochondrial membrane potential. Vitexin also significantly reduced the levels of p-PI3K, p-Akt and p-mTOR, and the pro-apoptotic effect of vitexin on A549 cells was partly blocked by SC79, an Akt activator.CONCLUSIONS:
Accordingly, we believed that vitexin could be used as a potential therapeutic agent for the treatment of NSCLC in the future.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Apoptosis
/
Carcinoma, Non-Small-Cell Lung
/
Phosphatidylinositol 3-Kinases
/
Apigenin
/
Proto-Oncogene Proteins c-akt
/
TOR Serine-Threonine Kinases
/
Lung Neoplasms
/
Antineoplastic Agents
Limits:
Animals
/
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2019
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Affiliated Cancer Hospital of Zhengzhou University/CN
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