MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
Biol. Res
;
52: 18, 2019. graf
Article
in English
| LILACS
| ID: biblio-1011420
ABSTRACT
BACKGROUND:
MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression.METHODS:
The levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were conducted to assess the roles of miR-663 in the migration and invasion of ovarian cancer cell in vitro. Rescue assays were carried out to confirm the contribution of tumor suppressor candidate 2 (TUSC2) in the aggressiveness of cancer cell which was regulated by miR-663.RESULTS:
The levels of miR-663 were up-regulated in ovarian cancer tissues in comparison with the corresponding normal tissues. Up-regulation of miR-663 increased the proliferation, colony formation, migration and invasion of ovarian cancer SKOV3 cell. Additional, over-expression of miR-663 increased the tumor growth of SKOV3 in xenograft model. Bioinformatics analysis and luciferase reporter assay identified that miR-663 decreased the level of TUSC2 via binding to the 3'-UTR of TUSC2 gene. Finally, the expression of TUSC2 was inversely associated with the level of miR-663 in ovarian carcinoma tissue and over-expression of TUSC2 inhibited the migration and invasion abilities of SKOV3 that was promoted by miR-663.CONCLUSION:
Altogether, these results indicate that miR-663 acts as a potential tumor-promoting miRNA through targeting TUSC2 in ovarian cancer.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ovarian Neoplasms
/
Tumor Suppressor Proteins
/
MicroRNAs
Limits:
Female
/
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2019
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
2nd Affiliated Hospital of Kunming Medical University/CN
/
Gynaecology Ward of Maternal and Child Health Hospital/CN
/
The Third Affiliated Hospital of Kunming Medical University/CN
/
Xuzhou Central Hospital/CN
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