Detection of Diarrheagenic Escherichia coli in Bovine Meat in the Northern Region of Paraná State, Brazil
Braz. arch. biol. technol
;
62: e19180012, 2019. tab
Article
in English
| LILACS
| ID: biblio-1011518
ABSTRACT
Abstract Ground bovine meat is commonly consumed by the population of Brazil. However, it constitutes an excellent medium for the multiplication of microorganisms due to available nutrients and handling practices prior to consumption. Here, we examined 100 samples of ground beef for the presence of diarrheagenic Escherichia coli (DEC) pathotypes by PCR, and characterized isolates by analyzing their adherence to HEp-2 cells, serotype, antimicrobial susceptibility, and phylogeny. Enteroaggregative E. coli was detected in five (5%) meat samples, Shiga toxin-producing E. coli in three (3%), and atypical enteropathogenic E. coli in two (2%). According to the phylogeny, six isolates (60%) were classified in group A, two (20%) in group B1, and two (20%) in group E. The detected serotypes were O3H2, O93H9, O93H46, O105abH7, O152H8, O156H10, and O175H7. The antimicrobial susceptibility testing showed that one sample (10%) was resistant to ampicillin, two (20%) to sulfamethoxazole-trimethoprim, and two (20%) to cephalothin. Based on these results, bovine ground meat for human consumption can serve as a reservoir of DEC, which emphasizes the importance of appropriate hygienic-sanitary conditions during handling at every stage from slaughter to table.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Escherichia coli
/
Red Meat
Type of study:
Diagnostic study
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2019
Type:
Article
Affiliation country:
Brazil
/
Mexico
Institution/Affiliation country:
State University of Londrina/BR
/
Universidad Nacional Autónoma de México/MX
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