Use of Inert Gases for the Preservation of Nuclear Blood Cells
Braz. arch. biol. technol
;
62: e19180204, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1011539
ABSTRACT
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte survival rate is 73.5±2.7%, original lipid peroxidation rate and antioxidant activity are retained. Biological object cryopreservation in noble gases environment is a promising trend in biology and medicine.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Xenon
/
Cryopreservation
/
Leukocytes
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2019
Type:
Article
Affiliation country:
RUSSIA
Institution/Affiliation country:
Russian Academy of Sciences/RU
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