Screening of Strongyloides infection using an ELISA test in transplant candidates
Clinics
;
74: e698, 2019. graf
Article
in English
| LILACS
| ID: biblio-1011925
ABSTRACT
OBJECTIVES:
Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates.METHODS:
An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen.RESULTS:
In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl.CONCLUSIONS:
The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Strongyloidiasis
/
Immunoglobulin G
/
Organ Transplantation
/
Strongyloides stercoralis
/
Antigens, Helminth
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2019
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Santa Casa/BR
/
Universidade de Sao Paulo/BR
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