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Role of extracellular DNA in Enterococcus faecalis biofilm formation and its susceptibility to sodium hypochlorite
YU, Mi-Kyung; KIM, Mi-Ah; ROSA, Vinicius; HWANG, Yun-Chan; DEL FABBRO, Massimo; SOHN, Won-Jun; MIN, Kyung-San.
  • YU, Mi-Kyung; Chonbuk National University. School of Dentistry and Institute of Oral Bioscience. Department of Conservative Dentistry. Jeonju. KR
  • KIM, Mi-Ah; Chonbuk National University. School of Dentistry and Institute of Oral Bioscience. Department of Conservative Dentistry. Jeonju. KR
  • ROSA, Vinicius; National University of Singapore. Faculty of Dentistry. SG
  • HWANG, Yun-Chan; Chonnam National University. School of Dentistry. Department of Conservative Dentistry. Gwangju. KR
  • DEL FABBRO, Massimo; Università degli Studi di Milano. Dipartimento di scienze biomediche, chirurgiche e odontoiatriche. Milan. IT
  • SOHN, Won-Jun; Seoul National University. Dental Research Institute and School of Dentistry. Department of Conservative Dentistry. Seoul. KR
  • MIN, Kyung-San; Chonbuk National University. School of Dentistry and Institute of Oral Bioscience. Department of Conservative Dentistry. Jeonju. KR
J. appl. oral sci ; 27: e20180699, 2019. graf
Article in English | LILACS, BBO | ID: biblio-1012504
ABSTRACT
Abstract Objective This study investigated the role of extracellular deoxyribonucleic acid (eDNA) on Enterococcus faecalis ( E. faecalis ) biofilm and the susceptibility of E. faecalis to sodium hypochlorite (NaOCl). Methodology E. faecalis biofilm was formed in bovine tooth specimens and the biofilm was cultured with or without deoxyribonuclease (DNase), an inhibitor of eDNA. Then, the role of eDNA in E. faecalis growth and biofilm formation was investigated using colony forming unit (CFUs) counting, eDNA level assay, crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy. The susceptibility of E. faecalis biofilm to low (0.5%) or high (5%) NaOCl concentrations was also analyzed by CFU counting. Results CFUs and biofilm formation decreased significantly with DNase treatment (p<0.05). The microstructure of DNase-treated biofilms exhibited less structured features when compared to the control. The volume of exopolysaccharides in the DNase-treated biofilm was significantly lower than that of control (p<0.05). Moreover, the CFUs, eDNA level, biofilm formation, and exopolysaccharides volume were lower when the biofilm was treated with DNase de novo when compared to when DNase was applied to matured biofilm (p<0.05). E. faecalis in the biofilm was more susceptible to NaOCl when it was cultured with DNase (p<0.05). Furthermore, 0.5% NaOCl combined with DNase treatment was as efficient as 5% NaOCl alone regarding susceptibility (p>0.05). Conclusions Inhibition of eDNA leads to decrease of E. faecalis biofilm formation and increase of susceptibility of E. faecalis to NaOCl even at low concentrations. Therefore, our results suggest that inhibition of eDNA would be beneficial in facilitating the efficacy of NaOCl and reducing its concentration.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Sodium Hypochlorite / DNA, Bacterial / Enterococcus faecalis / Biofilms / Deoxyribonucleases Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Italy / Singapore / South Korea Institution/Affiliation country: Chonbuk National University/KR / Chonnam National University/KR / National University of Singapore/SG / Seoul National University/KR / Università degli Studi di Milano/IT

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Full text: Available Index: LILACS (Americas) Main subject: Sodium Hypochlorite / DNA, Bacterial / Enterococcus faecalis / Biofilms / Deoxyribonucleases Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Italy / Singapore / South Korea Institution/Affiliation country: Chonbuk National University/KR / Chonnam National University/KR / National University of Singapore/SG / Seoul National University/KR / Università degli Studi di Milano/IT