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Composite-derived monomers affect cell viability and cytokine expression in human leukocytes stimulated with Porphyromonas gingivalis
Programa de Pós-graduação em OdontologiaNeves, Sheyla Omonte; Departmento de MorfologiaMagalhães, Luísa Mourão Dias; Programa de Pós-graduação em OdontologiaCorrêa, Jôice Dias; Departmento de MorfologiaDutra, Walderez Ornelas; Gollob, Kenneth John; Silva, Tarcília Aparecida; Programa de Pós-graduação em OdontologiaHorta, Martinho Campolina Rebello; Programa de Pós-graduação em OdontologiaSouza, Paulo Eduardo Alencar.
  • Programa de Pós-graduação em OdontologiaNeves, Sheyla Omonte; Pontifícia Universidade Católica de Minas Gerais. Departamento de Odontologia. Programa de Pós-graduação em OdontologiaNeves, Sheyla Omonte. Belo Horizonte. BR
  • Departmento de MorfologiaMagalhães, Luísa Mourão Dias; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departmento de MorfologiaMagalhães, Luísa Mourão Dias. Belo Horizonte. BR
  • Programa de Pós-graduação em OdontologiaCorrêa, Jôice Dias; Pontifícia Universidade Católica de Minas Gerais. Departamento de Odontologia. Programa de Pós-graduação em OdontologiaCorrêa, Jôice Dias. Belo Horizonte. BR
  • Departmento de MorfologiaDutra, Walderez Ornelas; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departmento de MorfologiaDutra, Walderez Ornelas. Belo Horizonte. BR
  • Gollob, Kenneth John; International Research Center. A.C.Camargo Cancer Center. São Paulo. BR
  • Silva, Tarcília Aparecida; Universidade Federal de Minas Gerais. Faculdade de Odontologia. Belo Horizonte. BR
  • Programa de Pós-graduação em OdontologiaHorta, Martinho Campolina Rebello; Pontifícia Universidade Católica de Minas Gerais. Departamento de Odontologia. Programa de Pós-graduação em OdontologiaHorta, Martinho Campolina Rebello. Belo Horizonte. BR
  • Programa de Pós-graduação em OdontologiaSouza, Paulo Eduardo Alencar; Pontifícia Universidade Católica de Minas Gerais. Departamento de Odontologia. Programa de Pós-graduação em OdontologiaSouza, Paulo Eduardo Alencar. Belo Horizonte. BR
J. appl. oral sci ; 27: e20180529, 2019. graf
Article in English | LILACS, BBO | ID: biblio-1012507
ABSTRACT
Abstract

Objectives:

Dental composites release unreacted resin monomers into the oral environment, even after polymerization. Periodontal cells are, therefore, exposed to substances that potentially elicit the immune inflammatory response. The underlying molecular mechanisms associated with the interaction between resin monomers and human immune cells found in the gingival crevicular fluid are not fully understood yet. This study investigated the ability of bisphenol A-glycidyl methacrylate (BISGMA), urethane dimethacrylate (UDMA) and triethylene glycol dimethacrylate (TEGDMA) to induce apoptosis and cytokine release by human leukocytes stimulated with a periodontal pathogen.

Methodology:

Peripheral blood mononuclear cells (PBMC) from 16 healthy individuals were included in this study. To determine the toxicity, the PBMC were incubated for 20 hours, with monomers, for the analysis of cell viability using MTT assay. To evaluate cell death in the populations of monocytes and lymphocytes, they were exposed to sub-lethal doses of each monomer and of heat-inactivated Porphyromonas gingivalis (P. gingivalis) for 5 hours. Secretions of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA after 20 hours.

Results:

UDMA and TEGDMA induced apoptosis after a short-time exposure. Bacterial challenge induced significant production of IL-1β and TNF-α (p<0.05). TEGDMA reduced the bacterial-induced release of IL-1β and TNF-α, whereas UDMA reduced IL-1β release (p<0.05). These monomers did not affect IL-10 and IL-6 secretion. BISGMA did not significantly interfere in cytokine release.

Conclusions:

These results show that resin monomers are toxic to PBMC in a dose-dependent manner, and may influence the local immune inflammatory response and tissue damage mechanisms via regulation of bacterial-induced IL-1β and TNF-α secretion by PBMC.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Polyethylene Glycols / Polymethacrylic Acids / Polyurethanes / Leukocytes, Mononuclear / Cytokines / Bisphenol A-Glycidyl Methacrylate / Porphyromonas gingivalis / Methacrylates Type of study: Evaluation studies Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: International Research Center/BR / Pontifícia Universidade Católica de Minas Gerais/BR / Universidade Federal de Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Polyethylene Glycols / Polymethacrylic Acids / Polyurethanes / Leukocytes, Mononuclear / Cytokines / Bisphenol A-Glycidyl Methacrylate / Porphyromonas gingivalis / Methacrylates Type of study: Evaluation studies Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: International Research Center/BR / Pontifícia Universidade Católica de Minas Gerais/BR / Universidade Federal de Minas Gerais/BR