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Proteomic analysis of excretory-secretory products from young adults of Angiostrongylus cantonensis
Chen, Kuang-Yao; Lu, Pei-Jhen; Cheng, Chien-Ju; Jhan, Kai-Yuan; Yeh, Shih-Chien; Wang, Lian-Chen.
  • Chen, Kuang-Yao; China Medical University. School of Medicine. Department of Parasitology. Taichung. TW
  • Lu, Pei-Jhen; Chang Gung University. College of Medicine. Department of Parasitology. Taoyuan. TW
  • Cheng, Chien-Ju; Chang Gung University. College of Medicine. Department of Parasitology. Taoyuan. TW
  • Jhan, Kai-Yuan; Chang Gung University. College of Medicine. Graduate Institute of Biomedical Sciences. Taoyuan. TW
  • Yeh, Shih-Chien; Chang Gung University. College of Medicine. Department of Parasitology. Taoyuan. TW
  • Wang, Lian-Chen; Chang Gung University. College of Medicine. Department of Parasitology. Taoyuan. TW
Mem. Inst. Oswaldo Cruz ; 114: e180556, 2019. tab, graf
Article in English | LILACS | ID: biblio-1012674
ABSTRACT
BACKGROUND Angiostrongyliasis is caused by the nematode Angiostrongylus cantonensis and can lead to eosinophilic meningitis and meningoencephalitis in humans. The young adult worms play central pathogenic roles in the central nervous system (CNS); however, the underlying mechanism is unclear. Excretory-secretory products (ESPs) are good investigation targets for studying the relationship between a host and its parasite. OBJECTIVES We aimed to profile, identify, and characterise the proteins in the ESPs of A. cantonensis young adults. METHODS The ESPs of young adult worms were collected from culture medium after incubation ranging from 24 to 96 h. Proteomic and bioinformatics analyses were performed to characterise the ESPs. FINDINGS A total of 51 spots were identified, and the highly expressed proteins included two protein disulphide isomerases, one calreticulin, and three uncharacterised proteins. Subsequently, approximately 254 proteins were identified in the ESPs of A. cantonensis young adults via liquid chromatography-mass spectrometry (LC-MS/MS) analysis, and these were further classified according to their characteristics and biological functions. Finally, we identified the immunoreactive proteins from a reference map of ESPs from A. cantonensis young adults. Approximately eight proteins were identified, including a protein disulphide isomerase, a putative aspartic protease, annexin, and five uncharacterised proteins. The study established and identified protein reference maps for the ESPs of A. cantonensis young adults. MAIN CONCLUSIONS The identified proteins may be potential targets for the development of diagnostic or therapeutic agents for human angiostrongyliasis.
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Full text: Available Index: LILACS (Americas) Main subject: Parasite Egg Count / Angiostrongylus cantonensis Type of study: Prognostic study Limits: Adolescent / Adult / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article / Project document Affiliation country: China / Taiwan Institution/Affiliation country: Chang Gung University/TW / China Medical University/TW

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Full text: Available Index: LILACS (Americas) Main subject: Parasite Egg Count / Angiostrongylus cantonensis Type of study: Prognostic study Limits: Adolescent / Adult / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article / Project document Affiliation country: China / Taiwan Institution/Affiliation country: Chang Gung University/TW / China Medical University/TW