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Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study
Beatty, Norman L; Behrens-Bradley, Nicole; Love, Maria; McCants, Finn; Smith, Shannon; Schmidt, Justin O; Hamer, Sarah A; Dorn, Patricia L; Ahmad, Nafees; Klotz, Stephen A.
  • Beatty, Norman L; University of Arizona College of Medicine. Department of Medicine. Division of Infectious Diseases. Tucson. US
  • Behrens-Bradley, Nicole; University of Arizona College of Medicine. Department of Immunobiology. Tucson. US
  • Love, Maria; University of Arizona College of Medicine. Department of Immunobiology. Tucson. US
  • McCants, Finn; Loyola University New Orleans. Department of Biological Sciences. New Orleans. US
  • Smith, Shannon; University of Arizona College of Medicine. Department of Medicine. Division of Infectious Diseases. Tucson. US
  • Schmidt, Justin O; Southwestern Biological Institute. Tucson,. US
  • Hamer, Sarah A; Texas A&M University. Veterinary Medicine and Biomedical Sciences. College Station. US
  • Dorn, Patricia L; Loyola University New Orleans. Department of Biological Sciences. New Orleans. US
  • Ahmad, Nafees; University of Arizona College of Medicine. Department of Immunobiology. Tucson. US
  • Klotz, Stephen A; University of Arizona College of Medicine. Department of Medicine. Division of Infectious Diseases. Tucson. US
Mem. Inst. Oswaldo Cruz ; 114: e190047, 2019. tab, graf
Article in English | LILACS | ID: biblio-1012677
ABSTRACT
BACKGROUND DNA- and proteomics-based techniques are currently used to identify a triatomine human blood meal. These methods are time consuming, require access to laboratories with sophisticated equipment, and trained personnel. OBJECTIVES We tested a rapid and specific immunochromatographic assay (that detects human blood in forensic samples) to determine if human blood was present in triatomines and their fecal excreta. METHODS We fed Triatoma rubida human blood (positive control) or mouse blood (negative control) and performed the assay on the abdominal contents and fecal excreta. Triatomine field specimens collected in and around human habitations and excreta were also tested. FINDINGS The assay was positive in triatomines fed human blood (N = 5/5) and fecal excreta from bugs known to have ingested human blood (N = 5/5). Bugs feeding on mice (N = 15/15) and their fecal excreta (N = 8/8) were negative for human blood. Human blood was detected in 47% (N = 23/49) triatomines, representing six different species, collected in the field. MAIN CONCLUSIONS The pilot study shows that this rapid and specific test may have applications in triatomine research. Further study is needed to determine the sensitivity of this assay compared to other well-established techniques, such as DNA- and proteomics-based methodologies and the assay's application in the field.
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Full text: Available Index: LILACS (Americas) Main subject: Immunoassay / Chromatography, Affinity / Triatominae Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article Affiliation country: United States Institution/Affiliation country: Loyola University New Orleans/US / Southwestern Biological Institute/US / Texas A&M University/US / University of Arizona College of Medicine/US

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Full text: Available Index: LILACS (Americas) Main subject: Immunoassay / Chromatography, Affinity / Triatominae Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article Affiliation country: United States Institution/Affiliation country: Loyola University New Orleans/US / Southwestern Biological Institute/US / Texas A&M University/US / University of Arizona College of Medicine/US