Improvement of clavulanic acid production in Streptomyces clavuligerus F613-1 by using a claR-neo reporter strategy
Electron. j. biotechnol
;
28: 41-46, July. 2017. tab, ilus, graf
Article
in English
| LILACS
| ID: biblio-1015839
ABSTRACT
Background:
Streptomyces clavuligerus was the producer of clavulanic acid, claR, a pathway-specific transcriptional regulator in S. clavuligerus, positively regulates clavulanic acid biosynthesis. In this study, the promoter-less kanamycin resistance gene neo was fused with claR to obtain strain NEO from S. clavuligerus F613-1. The claR-neo fusion strain NEO was mutated using physical and chemical mutagens and then screened under high concentrations of kanamycin for high-yield producers of clavulanic acid.Results:
The reporter gene neo was fused downstream of claR and used as an indicator for expression levels of claR in strain NEO. After three rounds of continuous treatment and screening, the high-yield clavulanic acid-producing strain M3-19 was obtained. In the shaking flask model, the clavulanic acid titer of M3-19 reached 4.33 g/L, which is an increase of 33% over the titer of 3.26 g/L for the starting strains S. clavuligerus F613-1 and NEO.Conclusions:
Our results indicate that neo can be effectively used as a reporter for the expression of late-stage biosynthetic genes when screening for high-yield strains and that this approach has strong potential for improving Streptomyces strains of industrial value.
Full text:
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Index:
LILACS (Americas)
Main subject:
Streptomyces
/
Kanamycin
/
Clavulanic Acid
Type of study:
Prognostic study
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2017
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Shandong Jianzhu University/CN
/
Shandong University/CN
/
University of Jinan-Shandong Academy of Medical Sciences/CN
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