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Mannheimia haemolytica growth and leukotoxin production for vaccine manufacturing: a bioprocess review
Oppermann, Tobias; Busse, Nadine; Czermak, Peter.
  • Oppermann, Tobias; Fraunhofer Institute for Molecular Biology and Applied Ecology. Giessen. DE
  • Busse, Nadine; University of Applied Sciences Mittelhessen. Institute of Bioprocess Engineering and Pharmaceutical Technology. Giessen. DE
  • Czermak, Peter; Fraunhofer Institute for Molecular Biology and Applied Ecology. Giessen. DE
Electron. j. biotechnol ; 28: 95-100, July. 2017. tab
Article in English | LILACS | ID: biblio-1016080
ABSTRACT
Mannheimia haemolytica leukotoxin (LKT) is a known cause of bovine respiratory disease (BRD) which results in severe economic losses in the cattle industry (up to USD 1 billion per year in the USA). Vaccines based on LKT offer the most promising measure to contain BRD outbreaks and are already commercially available. However, insufficient LKT yields, predominantly reflecting a lack of knowledge about the LKT expression process, remain a significant engineering problem and further bioprocess optimization is required to increase process efficiency. Most previous investigations have focused on LKT activity and cell growth, but neither of these parameters defines reliable criteria for the improvement of LKT yields. In this article, we review the most important process conditions and operational parameters (temperature, pH, substrate concentration, dissolved oxygen level, medium composition and the presence of metabolites) from a bioprocess engineering perspective, in order to maximize LKT yields.
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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Toxins / Mannheimia haemolytica / Bovine Respiratory Disease Complex / Exotoxins Limits: Animals Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article Affiliation country: Germany Institution/Affiliation country: Fraunhofer Institute for Molecular Biology and Applied Ecology/DE / University of Applied Sciences Mittelhessen/DE

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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Toxins / Mannheimia haemolytica / Bovine Respiratory Disease Complex / Exotoxins Limits: Animals Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article Affiliation country: Germany Institution/Affiliation country: Fraunhofer Institute for Molecular Biology and Applied Ecology/DE / University of Applied Sciences Mittelhessen/DE