Mannheimia haemolytica growth and leukotoxin production for vaccine manufacturing: a bioprocess review
Electron. j. biotechnol
;
28: 95-100, July. 2017. tab
Article
in English
| LILACS
| ID: biblio-1016080
ABSTRACT
Mannheimia haemolytica leukotoxin (LKT) is a known cause of bovine respiratory disease (BRD) which results in severe economic losses in the cattle industry (up to USD 1 billion per year in the USA). Vaccines based on LKT offer the most promising measure to contain BRD outbreaks and are already commercially available. However, insufficient LKT yields, predominantly reflecting a lack of knowledge about the LKT expression process, remain a significant engineering problem and further bioprocess optimization is required to increase process efficiency. Most previous investigations have focused on LKT activity and cell growth, but neither of these parameters defines reliable criteria for the improvement of LKT yields. In this article, we review the most important process conditions and operational parameters (temperature, pH, substrate concentration, dissolved oxygen level, medium composition and the presence of metabolites) from a bioprocess engineering perspective, in order to maximize LKT yields.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacterial Toxins
/
Mannheimia haemolytica
/
Bovine Respiratory Disease Complex
/
Exotoxins
Limits:
Animals
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2017
Type:
Article
Affiliation country:
Germany
Institution/Affiliation country:
Fraunhofer Institute for Molecular Biology and Applied Ecology/DE
/
University of Applied Sciences Mittelhessen/DE
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