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MiR-106a aggravates sepsis-induced acute kidney injury by targeting THBS2 in mice model
Shen, Yezhou; Yu, Jiaoyang; Jing, Yunyan; Zhang, Jian.
  • Shen, Yezhou; Hangzhou Normal University. Hangzhou. CN
  • Yu, Jiaoyang; Hangzhou Normal University. Hangzhou. CN
  • Jing, Yunyan; Hangzhou Normal University. Hangzhou. CN
  • Zhang, Jian; Hangzhou Normal University. Hangzhou. CN
Acta cir. bras ; 34(6): e201900602, 2019. tab, graf
Article in English | LILACS | ID: biblio-1019265
ABSTRACT
Abstract Purpose To investigate the role and related mechanisms of miR-106a in sepsis-induced AKI. Methods Serum from sepsis and healthy patients was collected, sepsis mouse model was established by cecal ligation and puncture (CLP). TCMK-1 cells were treated with lipopolysaccharide (LPS) and transfected with THBS2-small interfering RNA (siTHBS2), miR-106a inhibitor, miR-106a mimics and their negative controls (NCs). The expression of miR-106a, thrombospondin 2 (THBS2), Bax, cleaved caspase-3 and Bcl-2, cell viability, relative caspase-3 activity and TNF-α, IL-1β, IL-6 content were respectively detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, Cell Counting Kit-8 (CCK-8) and enzyme linked immunosorbent assay (ELISA). The relationship between miR-106a and THBS2 was confirmed by dual luciferase reporter assay. Results MiR-106a was up-regulated in serum of sepsis patients, CLP-induced mice models and LPS-induced TCMK-1 cells. LPS reduced cell viability and Bcl-2 expression, and increased caspase-3 activity, Bax expression, the content of TNF-α, IL-1β, IL-6. THBS2 was a target of miR-106a. The decreases of caspase-3 activity, TNF-α, IL-1β, IL-6, Bax expression and the increases of cell viability, Bcl-2 expression caused by miR-106a knockdown were reversed when THBS2 silencing in LPS-stimulated TCMK-1 cells. Conclusion MiR-106a aggravated LPS-induced inflammation and apoptosis of TCMK-1 cells via regulating THBS2 expression.
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Full text: Available Index: LILACS (Americas) Main subject: Sepsis / Thrombospondins / MicroRNAs / Epithelial Cells / Acute Kidney Injury / Kidney Type of study: Observational study Limits: Animals / Female / Humans / Male Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Hangzhou Normal University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Sepsis / Thrombospondins / MicroRNAs / Epithelial Cells / Acute Kidney Injury / Kidney Type of study: Observational study Limits: Animals / Female / Humans / Male Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Hangzhou Normal University/CN