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miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
Zhang, Bao; Shetti, Dattatrya; Fan, Conghui; Wei, Kun.
  • Zhang, Bao; South China University of Technology. School of Biology and Biological Engineering. Guangzhou. CN
  • Shetti, Dattatrya; South China University of Technology. School of Biology and Biological Engineering. Guangzhou. CN
  • Fan, Conghui; South China University of Technology. School of Biology and Biological Engineering. Guangzhou. CN
  • Wei, Kun; South China University of Technology. School of Biology and Biological Engineering. Guangzhou. CN
Biol. Res ; 52: 38, 2019. graf
Article in English | LILACS | ID: biblio-1019502
ABSTRACT

BACKGROUND:

Breast cancer is the second common malignant cancer among females worldwide. Accumulating studies have indicated that deregulation of miRNA expression in breast cancer will contribute to tumorigenesis and form different cancer subtypes. However, the reported studies on miR-29b-3p-regulated breast cancer are limited so far. Herein, we investigated the role and mechanism of miR-29b-3p in the triple negative breast cancer cell line MDA-MB-231.

METHODS:

The relative miR-29b-3p expression in different breast cancer cell lines were determined by qRT-PCR. CCK8 and colony formation assay were used to determine the influence of miR-29b-3p on cell proliferation. Migration assay and invasion assay were performed for cell migration and invasion respectively. To study the cell integrity immunofluorescence was performed. TUNEL assay, flow cytometry assay, hoechst staining and western blot were conducted to determine the influence of miR-29b-3p inhibitor on cell apoptosis. TRAF3 was found to be the target gene of miR-29b-3p using bioinformatics predictions. Dual-luciferase assay was performed to determine the relative luciferase activity in NC, miR-29b-3p mimic, miR-29b-3p inhibitor with TRAF3 3'-UTR wt or TRAF3 3'-UTR mt reporter plasmids. The proteins expression of NF-κB signaling pathway in MDA-MB-231 after transfection with NC, miR-29b-3p mimic, miR-29b-3p inhibitor were determined by western blot.

RESULTS:

The miR-29b-3p expression was significantly increased in MDA-MB-231 compare with MCF-10A. miR-29b-3p inhibitor reduced the cell viability of MDA-MB-231 and inhibited cell migration and invasion. Cell cytoskeleton integrity destroyed after miR-29b-3p inhibitor treatment. Furthermore, we identified the mechanism and found miR-29b-3p targets the TRAF3 and activates NF-κB signaling pathway.

CONCLUSIONS:

From the above studies, our results indicated that miR-29b-3p acts as a promoter for the development of MDA-MB-231.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Down-Regulation / Apoptosis / MicroRNAs / TNF Receptor-Associated Factor 3 / Triple Negative Breast Neoplasms Type of study: Prognostic study Limits: Female / Humans Language: English Journal: Biol. Res Journal subject: Biology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: South China University of Technology/CN

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Full text: Available Index: LILACS (Americas) Main subject: Down-Regulation / Apoptosis / MicroRNAs / TNF Receptor-Associated Factor 3 / Triple Negative Breast Neoplasms Type of study: Prognostic study Limits: Female / Humans Language: English Journal: Biol. Res Journal subject: Biology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: South China University of Technology/CN