Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L)
Braz. arch. biol. technol
;
62: e19180228, 2019. tab
Article
in English
| LILACS
| ID: biblio-1019546
ABSTRACT
Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars within 8 weeks. The somatic embryos developed into rooted plants with a 100% survival rate upon transfer to the greenhouse.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plant Growth Regulators
/
Seeds
/
Silver Nitrate
/
Coffea
Type of study:
Evaluation studies
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2019
Type:
Article
Affiliation country:
Brazil
/
Costa Rica
Institution/Affiliation country:
Agronomic Institute of Paraná/BR
/
University of Costa Rica/CR
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