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Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy
Arrym, Mauro Pedromonico; Alves, Paulo César Martins; Castelhano, Mariana Virginello; Mazzola, Taís Nitsch; Lemos, Renata Muller Banzato Pinto de; Zaccariotto, Tânia Regina; Levy, Carlos Emilio; Guimarães, Fernando; Silva, Marcos Tadeu Nolasco da.
  • Arrym, Mauro Pedromonico; State University of Campinas. Faculty of Medical Sciences. Laboratory of Translational Research in Child and Adolescent Health. Campinas. BR
  • Alves, Paulo César Martins; State University of Campinas. Faculty of Medical Sciences. Laboratory of Translational Research in Child and Adolescent Health. Campinas. BR
  • Castelhano, Mariana Virginello; State University of Campinas. Faculty of Medical Sciences. Laboratory of Translational Research in Child and Adolescent Health. Campinas. BR
  • Mazzola, Taís Nitsch; State University of Campinas. Center for Molecular Biology and Genetical Engineering. Campinas. BR
  • Lemos, Renata Muller Banzato Pinto de; State University of Campinas. Clinics Hospital. Campinas. BR
  • Zaccariotto, Tânia Regina; State University of Campinas. Clinics Hospital. Clinical Pathology Laboratory. Campinas. BR
  • Levy, Carlos Emilio; State University of Campinas. Clinics Hospital. Clinical Pathology Laboratory. Campinas. BR
  • Guimarães, Fernando; State University of Campinas. Center for Integral Attention to Women s Health. BR
  • Silva, Marcos Tadeu Nolasco da; State University of Campinas. Faculty of Medical Sciences. Laboratory of Translational Research in Child and Adolescent Health. Campinas. BR
Braz. j. infect. dis ; 23(3): 151-159, May-June 2019. tab
Article in English | LILACS | ID: biblio-1019551
Responsible library: BR1.1
ABSTRACT
ABSTRACT

Background:

HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents.

Methods:

We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers.

Results:

TCR γδ T-cell CD107a expression was similar between groups in response to mycobacterial antigens, and lower in the HIV-infected group in response to mitogen. Higher baseline HLA-DR expression and lower mycobacterial-stimulated expression was found within the HIV-infected group.

Conclusions:

Similar degranulation in stimulated CD8+ and TCR γδ T-cells from HIV-infected adolescents, when compared to healthy controls suggests long-term immunological preservation with immune reconstitution under successful cART. However, differences in HLA-DR expression may represent ongoing inflammation and lower specific responses in HIV-infected youth. These features may be relevant in the context of the precocity and severity of vertically acquired HIV infection.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Receptors, Antigen, T-Cell, alpha-beta / AIDS-Related Opportunistic Infections / CD8-Positive T-Lymphocytes / Anti-HIV Agents / Mycobacterium tuberculosis / Antigens, Bacterial Limits: Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: State University of Campinas/BR

Full text: Available Index: LILACS (Americas) Main subject: Receptors, Antigen, T-Cell, alpha-beta / AIDS-Related Opportunistic Infections / CD8-Positive T-Lymphocytes / Anti-HIV Agents / Mycobacterium tuberculosis / Antigens, Bacterial Limits: Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: State University of Campinas/BR