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Influence of photobiomodulation therapy on root development of rat molars with open apex and pulp necrosis
Zaccara, Ivana Maria; Jardine, Alexander Pompermayer; Mestieri, Letícia Boldrin; Quintana, Ramiro Martins; Jesus, Luciano; Mereira, Maria Stella; Grecca, Fabiana Soares; Martins, Manoela Domingues; Kopper, Patrícia Maria Poli.
  • Zaccara, Ivana Maria; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Jardine, Alexander Pompermayer; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Mestieri, Letícia Boldrin; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Quintana, Ramiro Martins; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Jesus, Luciano; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Mereira, Maria Stella; Universidade Ibirapuera. School of Dentistry. São Paulo. BR
  • Grecca, Fabiana Soares; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Martins, Manoela Domingues; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
  • Kopper, Patrícia Maria Poli; Universidade Federal do Rio Grande do Sul. School of Dentistry. Graduate program. Porto Alegre. BR
Braz. oral res. (Online) ; 33: e084, 2019. graf
Article in English | LILACS | ID: biblio-1019612
ABSTRACT
Abstract This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6) mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex.
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Full text: Available Index: LILACS (Americas) Main subject: Tooth Diseases / Dental Pulp Necrosis / Tooth Apex / Low-Level Light Therapy / Dental Pulp Cavity / Lasers, Semiconductor / Apexification Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR / Universidade Ibirapuera/BR

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Full text: Available Index: LILACS (Americas) Main subject: Tooth Diseases / Dental Pulp Necrosis / Tooth Apex / Low-Level Light Therapy / Dental Pulp Cavity / Lasers, Semiconductor / Apexification Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR / Universidade Ibirapuera/BR