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Susceptibilidad antimicrobiana y mutaciones en el gen ARNr 23S de Helicobacter pylori en pacientes dispépticos / Antimicrobial susceptibility and mutations in the 23S rRNA gen of Helicobacter pylori in dyspeptic patients
Guzmán, Jesús; Castillo, Denis; Ojeda, Manuel; Sauvain, Michel.
  • Guzmán, Jesús; Universidad Peruana Cayetano Heredia. Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida. Lima. PE
  • Castillo, Denis; Universidad Peruana Cayetano Heredia. Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida. Lima. PE
  • Ojeda, Manuel; Clínica Médica Cayetano Heredia. Lima. PE
  • Sauvain, Michel; Université de Toulouse. Toulouse. FR
Rev. peru. med. exp. salud publica ; 36(2): 270-274, abr.-jun. 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-1020780
RESUMEN
RESUMEN Con el objetivo de evaluar la susceptibilidad antimicrobiana y detectar mutaciones puntuales en el gen ARNr 23S en cepas de Helicobacter pylori se realizó un estudio transversal que incluyó a 95 pacientes con dispepsia atendidos en una clínica privada de Lima. Mediante endoscopía se colectaron biopsias de antro para el aislamiento de cepas de Helicobacter pylori para la evaluación de la susceptibilidad antimicrobiana empleando la técnica de microdilución en caldo. La detección de mutaciones puntuales se desarrolló mediante PCR-RFLP. El porcentaje de infección por Helicobacter pylori fue de 46,3%, se observaron valores de resistencia de 52,3% a claritromicina, 29,6% a metronidazol, 45,5% a levofloxacino y 4,6% a amoxicilina. El porcentaje de mutaciones puntuales A2142G y A2143G asociados a resistencia a claritromicina fue 43,5%. En conclusión, encontramos que las tasas de resistencia antimicrobiana y el porcentaje de cepas de Helicobacter pylori circulantes en una clínica privada de Lima fueron elevadas.
ABSTRACT
ABSTRACT In order to evaluate antimicrobial susceptibility and detect specific mutations in the 23S rRNA gene in Helicobacter pylori strains, a cross-sectional study was performed on 95 patients with dyspepsia treated in a private clinic in Lima. Antrum biopsies were collected by endoscopy for isolation and evaluation of antimicrobial susceptibility using the broth microdilution method. The detection of specific mutations was developed by PCR-RFLP. The percentage of infection by Helicobacter pylori was 46.3%. Resistance values of 52.3% to clarithromycin, 29.6% to metronidazole, 45.5% to levofloxacin, and 4.6% to amoxicillin were observed. The percentage of specific A2142G and A2143G mutations associated with clarithromycin resistance was 43.5%. In conclusion, we found that antimicrobial resistance rates and the percentage of Helicobacter pylori strains circulating in a private clinic in Lima were high.
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Full text: Available Index: LILACS (Americas) Main subject: Helicobacter pylori / Helicobacter Infections / Dyspepsia / Anti-Bacterial Agents Type of study: Observational study / Prevalence study / Risk factors Limits: Humans Country/Region as subject: South America / Peru Language: Spanish Journal: Rev. peru. med. exp. salud publica Journal subject: Public Health Year: 2019 Type: Article / Project document Affiliation country: France / Peru Institution/Affiliation country: Clínica Médica Cayetano Heredia/PE / Universidad Peruana Cayetano Heredia/PE / Université de Toulouse/FR

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Full text: Available Index: LILACS (Americas) Main subject: Helicobacter pylori / Helicobacter Infections / Dyspepsia / Anti-Bacterial Agents Type of study: Observational study / Prevalence study / Risk factors Limits: Humans Country/Region as subject: South America / Peru Language: Spanish Journal: Rev. peru. med. exp. salud publica Journal subject: Public Health Year: 2019 Type: Article / Project document Affiliation country: France / Peru Institution/Affiliation country: Clínica Médica Cayetano Heredia/PE / Universidad Peruana Cayetano Heredia/PE / Université de Toulouse/FR