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Correlación entre el grado de neoplasia intraepitelial cervical y la expresión dual de p16 Y ki67, en biopsia de cuello uterino en el Hospital Santa Rosa de Lima-Perú año 2016 / Correlation between the degree of cervical intraepitelial neoplasis (nic) and the dual expression of p16 and ki67, in uterine neck biopsy in Santa Rosa Hospital of Lima-Peru year 2016
Ortiz Muchotrigo, Nazario A; Torres Solano, Jaime L; Obando Corzo, Marilú F; Fabian Manzano, Judith E.
  • Ortiz Muchotrigo, Nazario A; Universidad Peruana Cayetano Heredia. Lima. PE
  • Torres Solano, Jaime L; Hospital Santa Rosa. Servicio de Ginecología Oncologíca. Lima. PE
  • Obando Corzo, Marilú F; Hospital Regional Ayacucho. Departamento de Ginecoobstetricia. Ayacucho. PE
  • Fabian Manzano, Judith E; Hospital Santa Rosa. Oficina de Epidemiología. Lima. PE
Rev. méd. panacea ; 6(3): 90-93, sept.-dic. 2017. ilus, tab
Article in Spanish | LILACS | ID: biblio-1022081
RESUMEN

Objetivo:

Determinar la frecuencia de coexpresión dual del p16 (INK4a) y ki67 en los diferentes grados de las neoplasias intraepiteliales cervicales (NIC) y su contribución molecular para diagnóstico y pronóstico. Material y

Metodos:

El diseño es descriptivo, prospectivo transversal. Se revisaron 20 biopsias de cérvix embebidas en parafina, que se encontraban en el archivo de Anatomía Patológica de un Hospital tercer nivel de Perú entre Enero y Diciembre del 2016, cuyas láminas portaobjeto fueron coloreadas con hematoxilina-eosina y reportadas como neoplasia intraepitelial (NIC) I, II, III. Se realizaron nuevos cortes histológicos de 3 micras para estudios complementarios usando la técnica de inmunohistoquímica dual. Las células con positividad para p16 mostraron tinción citoplasmática y/o nuclear de color rojizo y aquellas con positividad para ki67 mostraron coloración nuclear marrón.

Resultados:

Los 20 casos de neoplasia intraepitelial se dividen en 06 casos de NIC I; 11 casos de NIC II y 03 casos de NIC III. La tinción dual para el Ki67 y p16 se realizó a los 20 casos, esto mostró una coexpresión en el 85% y ausencia de expresión dual en 15%. Se observó tinción en los diversos grados de NIC predominando el grado 1+ en un 50%. En el grado 2+ y 3+ la expresión fuerte en NIC II y NIC III respectivamente. La expresión del Ki67 se dio en todos los diferentes grados de NIC.

Conclusiones:

La coexpresión del Ki67 y p16 es de 85% en los diversos grados de NIC, siendo frecuente en el grupo de NIC II. (AU)
ABSTRACT
Objetive To determine the frequency of dual coexpression of p16 (INK4a) and ki67 in the different degrees of cervical intraepithelial neoplasia (CIN) and its molecular contribution for diagnosis and prognosis. Material And

Methods:

the design is descriptive, prospective and cross-sectional. 20 cervix biopsies embedded in paraffin have been reviewed. These slides gathered from January 2016 to December 2016 were located in the records of Anatomical Pathology Department of a third level Hospital in Peru. The slides were stained with hematoxylin-eosin and reported as intraepithelial neoplasia (CIN) I, II, III. New histological sections of 3 microns were made for complementary studies by using dual immunohistochemistry technique. The cells with positivity for p16 showed cytoplasmic and / or nuclear reddish staining and those with positivity for ki67 showed brown nuclear coloration.

Results:

The 20 cases of intraepithelial neoplasia are divided into 06 cases of CIN I; 11 cases of CIN II and 03 cases of CIN III. Dual staining for Ki67 and p16 was performed in all 20 cases. This showed coexpression in 85% and absence of dual expression in 15%. Staining was observed in the different grades of CIN, with predominance in grade 1+ ( 50%). In grade 2+ and 3+ the expression was strong in CIN II and CIN III respectively. The expression of Ki67 occurred in all the different grades of NIC.

Conclusions:

The coexpression of Ki67 and p16 is 85% in the different grades of CIN, being frequent in the group of CIN II. (AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Carcinoma in Situ / Uterine Cervical Neoplasms / Hematoxylin Type of study: Observational study / Prevalence study / Risk factors Limits: Female / Humans Country/Region as subject: South America / Peru Language: Spanish Journal: Rev. méd. panacea Journal subject: Medicine Year: 2017 Type: Article Affiliation country: Ecuador / Peru Institution/Affiliation country: Hospital Regional Ayacucho/PE / Hospital Santa Rosa/PE / Universidad Peruana Cayetano Heredia/PE

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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma in Situ / Uterine Cervical Neoplasms / Hematoxylin Type of study: Observational study / Prevalence study / Risk factors Limits: Female / Humans Country/Region as subject: South America / Peru Language: Spanish Journal: Rev. méd. panacea Journal subject: Medicine Year: 2017 Type: Article Affiliation country: Ecuador / Peru Institution/Affiliation country: Hospital Regional Ayacucho/PE / Hospital Santa Rosa/PE / Universidad Peruana Cayetano Heredia/PE