Comparative study of the accuracy of different technique for the laboratory diagnosis of schistosomiasis mansoni in areas of low endemicity in Barra Mansa City, Rio de Janeiro State, Brazil
Biomed Res Int
;
2015: 1-16, 2015. ilus
Article
in English
| SES-SP, LILACS, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: biblio-1022429
ABSTRACT
Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (8295.5%), differed significantly from COPT in positivity , and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosomiasis mansoni
/
Aged, 80 and over
/
Brazil
/
Aged
/
Humans
/
Immunoassay
/
Precipitin Tests
/
Child
/
Child, Preschool
/
Population Surveillance
Type of study:
Controlled clinical trial
/
Diagnostic study
/
Etiology study
/
Prevalence study
/
Risk factors
/
Screening study
Limits:
Aged80
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Biomed Res Int
Year:
2015
Type:
Article
Institution/Affiliation country:
Secretaria da Saúde do Estado de São Paulo/BR
/
Universidade de São Paulo/BR
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