Pretentious genomic selection signatures in CYP19A1 gene associated with silent estrous behavior in water buffalo in Pakistan
Electron. j. biotechnol
;
32: 35-40, Mar. 2018. ilus, tab
Article
in English
| LILACS
| ID: biblio-1022620
ABSTRACT
Background:
Poor reproductive efficiency of river buffalos hampers the production capabilities of animals. Buffalos are mainly considered poor breeders owing to the constrained expression of estrus behavior. Failure to display heat signs is an indication of improper functionality of signaling peptides to trigger on a series of behavioral changes, which can be detectable by breeders for timely insemination of females. This might cause an animal to be a repeat breeder. Genomic variations underlying synthesis of signaling peptides can be a useful marker to select superior animals with better reproductive efficiency. In this context, the current study was designed to analyze the CYP19A1 gene in Nili-Ravi buffalo.Results:
A total of 97 animals were selected and were divided into two groups on the basis of their heat score. PCR amplification and sequencing of the amplicons were performed using the specific sets of primer, and then, sequences were analyzed for novel variants. A total of 11 polymorphic sites were identified illustrating phenotypic variation in the heat score. Most of the loci were found homologous. Single Nucleotide Polymorphisms (SNPs) were analyzed for association with silent estrus. A three-dimensional protein model was also generated to locate the position of exonic SNPs.Conclusion:
This study illustrated that polymorphic sites in the CYP19A1 gene provided potential markers for selection of buffalos with better estrus behavior.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Estrus
/
Buffaloes
/
Aromatase
/
Cytochrome P-450 Enzyme System
Type of study:
Prognostic study
/
Risk factors
Limits:
Animals
/
Pregnancy
Country/Region as subject:
Asia
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2018
Type:
Article
Affiliation country:
Pakistan
Institution/Affiliation country:
University of Veterinary & Animal Sciences/PK
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