Effects of cyclosporine on ischemia-reperfusion injuries in rat kidneys. An experimental model
Acta cir. bras
;
34(8): e201900806, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1038123
ABSTRACT
Abstract Purpose To assess Cyclosporine A (CsA) therapy at an intraperitoneal dose of 15 mg.kg -1 in a rodent model of non-septic renal ischemia. Methods Twenty male Wistar rats were randomized to receive CsA therapy or none therapy before undergoing 30 minutes of renal ischemia followed by reperfusion. Additionally, 10 rats were randomized to undergo the same surgical procedure of the aforementioned animals with neither ischemia nor CsA therapy. Twelve hours after kidney ischemia, the left kidneys were evaluated for histological injury according to Park's criteria. Serum creatinine (Cr), urea nitrogen (Ur) and sodium levels were obtained at different times of the experimental protocol. Results Rodents in the CsA group showed negative results (p<0.05) in serum variables (Cr 0.41±0.05mg/dL vs . 4.17±1.25mg/dL; Ur 40.90±3.98mg/dL vs . 187.70±22.93mg/dL) even the non CsA or control group (Cr 0.35±0.07mg/dL vs . 3.80±1.20mg/dL; Ur 40.10±4.70mg/dL vs . 184.50±49.80mg/dL). The negative results were also verified in histological evaluation, CsA group had 50% in the very severe grade of lesion, 10% in the severe and 40% in the moderate to severe whereas the control group had 90% in the very severe grade. Conclusion CsA was incapable of preventing the deleterious effects of ischemia-reperfusion injury in rat kidneys.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Reperfusion Injury
/
Cyclosporine
/
Immunosuppressive Agents
/
Kidney
Type of study:
Practice guideline
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UNESP/BR
/
Universidade Estadual Paulista Júlio de Mesquita Filho/BR
/
Universidade Federal da Bahia/BR
/
Universidade de Peranambuco/BR
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