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Acute Physical Stress Preconditions the Heart Against Ischemia/Reperfusion Injury Through Activation of Sympathetic Nervous System / Estresse Físico Agudo Precondiciona o Coração Contra a Injúria por Isquemia/Reperfusão Através da Ativação do Sistema Nervoso Simpático
Imani, Alireza; Parsa, Hoda; Chookalaei, Leila Gholami; Rakhshan, Kamran; Golnazari, Masoomeh; Faghihi, Mahdieh.
  • Imani, Alireza; Tehran University of Medical Sciences. School of Medicine. Department of physiology. Tehran. IR
  • Parsa, Hoda; Tehran University of Medical Sciences. School of Medicine. Department of physiology. Tehran. IR
  • Chookalaei, Leila Gholami; Tehran University of Medical Sciences. School of Medicine. Department of physiology. Tehran. IR
  • Rakhshan, Kamran; Iran University of Medical Sciences. School of Medicine. Department of physiology. Tehran. IR
  • Golnazari, Masoomeh; Hamedan Branch of Islamic Azad University. Basic Sciences faculty. Biology Department. Hamedan. IR
  • Faghihi, Mahdieh; Tehran University of Medical Sciences. School of Medicine. Department of physiology. Tehran. IR
Arq. bras. cardiol ; 113(3): 401-408, Sept. 2019. graf
Article in English | LILACS | ID: biblio-1038546
ABSTRACT
Abstract

Background:

Stress is defined as a complicated state that related to homeostasis disturbances, over-activity of the sympathetic nervous system and hypothalamus-pituitary-adrenal axis responses. Cardiac preconditioning reduces myocardial damages.

Objective:

This study was designed to assess the cardioprotective effects of acute physical stress against ischemia/reperfusion (I/R) injury through the activation of the sympathetic nervous system.

Methods:

Thirty-two male Wistar rats were divided into four groups; (1) IR (n = 8) rats underwent I/R, (2) Acute stress (St+IR) (n = 8) physical stress induced 1-hour before I/R, (3) Sympathectomy (Symp+IR) (n = 8) chemical sympathectomy was done 24-hours before I/R and (4) Sympathectomy- physical stress (Symp+St+IR) (n = 8) chemical sympathectomy induced before physical stress and I/R. Chemical sympathectomy was performed using 6-hydroxydopamine (100 mg/kg, sc). Then, the hearts isolated and located in the Langendorff apparatus to induce 30 minutes ischemia followed by 120 minutes reperfusion. The coronary flows, hemodynamic parameters, infarct size, corticosterone level in serum were investigated. P < 0.05 demonstrated significance.

Results:

Physical stress prior to I/R could improve left ventricular developed pressure (LVDP) and rate product pressure (RPP) of the heart respectively, (63 ± 2 versus 42 ± 1.2, p < 0.05, 70 ± 2 versus 43 ± 2.6, p < 0.05) and reduces infarct size (22.16 ± 1.3 versus 32 ± 1.4, p < 0.05) when compared with the I/R alone. Chemical sympathectomy before physical stress eliminated the protective effect of physical stress on I/R-induced cardiac damages (RPP 21 ± 6.6 versus 63 ± 2, p < 0.01) (LVDP 38 ± 4.5 versus 43 ± 2.6, p < 0.01) (infarct size 35 ± 3.1 versus 22.16 ± 1.3, p < 0.01).

Conclusion:

Findings indicate that acute physical stress can act as a preconditional stimulator and probably, the presence of sympathetic nervous system is necessary.
RESUMO
Resumo Fundamento O estresse é definido como um estado complicado de distúrbios da homeostase, hiperatividade do sistema nervoso simpático e das respostas do eixo hipotálamo-hipófise-adrenal. O pré-condicionamento cardíaco diminui os danos do miocárdio.

Objetivo:

Esse estudo avaliou os efeitos cardioprotetores do estresse físico agudo contra a lesão por isquemia-reperfusão (I/R) através da ativação do sistema nervoso simpático.

Métodos:

Trinta e dois ratos machos Wistar foram divididos em quatro grupos; (1) IR (n = 8) ratos submetidos a I/R, (2) Estresse agudo (St+IR) (n = 8) estresse físico induzido 1 hora antes da I/R, (3) Simpatectomia (Symp+IR) (n = 8) a simpatectomia química foi realizada 24 horas antes da I/R e (4) Simpatectomia-estresse físico (Symp+St+IR) (n = 8) simpatectomia induzida antes do estresse físico e da I/R. A simpatectomia química foi realizada com 6-hidroxidopamina (100 mg/kg, SC). Em seguida, os corações foram isolados e colocados em aparato de Lagendorff por 30 minutos para induzir isquemia, seguida de reperfusão por 120 minutos. Os fluxos coronarianos, os parâmetros hemodinâmicos, o tamanho do infarto e os níveis de corticosterona plasmática foram investigados. Valores de p < 0,05 foram considerados significativos.

Resultados:

O estresse físico anterior à I/R pode melhorar a pressão desenvolvida no ventrículo esquerdo (PDVE) e duplo produto (DP), respectivamente, (63 ± 2 versus 42 ± 1,2, p < 0,05, 70 ± 2 versus 43 ± 2,6, p < 0,05) e reduzir o tamanho do infarto (22,16 ± 1,3 versus 32±1,4, p < 0,05) quando comparado com a I/R isoladamente. A simpatectomia química antes do estresse físico eliminou o efeito protetor do estresse físico sobre os danos cardíacos induzidos pela I/R (DP 21 ± 6,6 versus 63 ± 2, p < 0,01) (PDVE 38 ± 4,5 versus 43 ± 2,6, p < 0,01) (tamanho do infarto 35 ± 3,1 versus 22,16 ± 1,3, p < 0,01).

Conclusão:

Os achados indicam que o estresse físico agudo pode funcionar como um estimulador pré-condicional e, provavelmente, a presença do sistema nervoso simpático é necessária.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Sympathetic Nervous System / Ischemic Preconditioning, Myocardial / Heart / Myocardial Infarction Limits: Animals Language: English Journal: Arq. bras. cardiol Journal subject: Cardiology Year: 2019 Type: Article Affiliation country: Iran Institution/Affiliation country: Hamedan Branch of Islamic Azad University/IR / Iran University of Medical Sciences/IR / Tehran University of Medical Sciences/IR

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Full text: Available Index: LILACS (Americas) Main subject: Sympathetic Nervous System / Ischemic Preconditioning, Myocardial / Heart / Myocardial Infarction Limits: Animals Language: English Journal: Arq. bras. cardiol Journal subject: Cardiology Year: 2019 Type: Article Affiliation country: Iran Institution/Affiliation country: Hamedan Branch of Islamic Azad University/IR / Iran University of Medical Sciences/IR / Tehran University of Medical Sciences/IR