Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)
Biol. Res
;
51: 42, 2018. tab
Article
in English
| LILACS
| ID: biblio-1038782
ABSTRACT
BACKGROUND:
Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement.RESULTS:
Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively.CONCLUSIONS:
From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.
Full text:
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Index:
LILACS (Americas)
Main subject:
DNA, Plant
/
Microsatellite Repeats
/
Gaultheria
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2018
Type:
Article
Affiliation country:
Chile
/
Ecuador
/
Singapore
Institution/Affiliation country:
BioTECNOS Ltda/CL
/
Pontificia Universidad Católica de Chile/CL
/
Universidad Católica del Maule/CL
/
Universidad Técnica de Manabí/EC
/
Verdant BioScience Pte. Ltda/SG
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