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Comparison of independent and dependent culture methods for the detection of transient bacteremia in diabetic subjects with chronic periodontitis / Comparación entre métodos de cultivo independientes y dependientes para la detección de bacteriemia transitoria en individuos diabéticos con periodontitis crónica
Ratto-Tespestini, Anna Carolina; Pérez-Chaparro, Paula Juliana; Romito, Guiseppe Alexandre; Figueiredo, Luciene Cristina; Faveri, Marcelo; Carillo, Hilana Paula; Larcher, Priscila; Feres, Magda.
  • Ratto-Tespestini, Anna Carolina; University of São Paulo. School of Dentistry. Department of Stomatology.
  • Pérez-Chaparro, Paula Juliana; Guarulhos University. Dental Research Division. Department of Periodontology.
  • Romito, Guiseppe Alexandre; University of São Paulo. School of Dentistry. Department of Stomatology.
  • Figueiredo, Luciene Cristina; Guarulhos University. Dental Research Division. Department of Periodontology.
  • Faveri, Marcelo; Guarulhos University. Dental Research Division. Department of Periodontology.
  • Carillo, Hilana Paula; University of São Paulo. School of Dentistry. Department of Stomatology.
  • Larcher, Priscila; University of São Paulo. Biological Medial Science Institute (ICB). São Paulo. BR
  • Feres, Magda; University of São Paulo. School of Dentistry. Department of Stomatology.
Biomédica (Bogotá) ; 36(1): 156-161, ene.-mar. 2016. tab
Article in English | LILACS | ID: biblio-1038784
ABSTRACT

Introduction:

Oral-derived bacteremia may occur after several dental procedures and routine daily activities. Some conditions of the oral cavity may favor episodes of bacteremia. This would be the case of patients with diabetes mellitus and periodontitis, who exhibit exacerbated gingival inflammation and may be more prone to developing oral-derived bacteremia.

Objective:

To compare the effectiveness of an independent culture method (quantitative real-time PCR- qCR) and the most commonly used method (BacT-ALERT 3D ® ) for the diagnosis of bacteremia. Materials and

methods:

Blood samples were drawn from subjects with type 2 diabetes mellitus and chronic periodontitis before and after apple chewing. Samples were processed by an automated blood culture system (BacT-ALERT 3D ® ) monitored for 15 days with suitable subculture of positive cultures. In parallel, whole DNA from blood samples was purified using a commercial kit and screened by qPCR using a universal primer set of 16S rDNA for bacteria detection.

Results:

Blood cultures taken before apple chewing were shown to be negative by the two diagnostic methods. After chewing, two samples (11%) showed bacterial growth by BacT-ALERT 3D ® whereas qPCR did not detect the presence of bacteria in any sample.

Conclusions:

qPCR did not show greater effectiveness than the BacT-ALERT 3D ® in the detection of bacteremia of oral origin.
RESUMEN
Introducción. Las bacteriemias de origen oral pueden ocurrir después de procedimientos odontológicos y de otros actos cotidianos. Algunas condiciones de la cavidad oral favorecen las bacteriemias como en el caso de pacientes con diabetes mellitus y periodontitis que presentan inflamación gingival exacerbada. Objetivo. Comparar la eficacia de un método independiente de cultivo (PCR cuantitativa) y otro dependiente (BacT-ALERT 3D ® ) en la detección de la bacteriemia. Materiales y métodos. Se tomaron muestras de sangre de individuos con diabetes mellitus de tipo II y periodontitis, antes y después de la masticación de manzana. Una alícuota se procesó por el sistema automatizado de hemocultivo (BacT-ALERT 3D ® ) y se monitorizó durante 15 días; la otra alícuota fue tratada para la extracción del ADN y procesada por RT-PCR usando un conjunto de cebadores de 16S rDNA exclusivos para bacterias. Resultados. En las muestras tomadas antes de masticar se confirmó la ausencia de bacterias mediante los dos métodos. En las muestras tomadas después de masticar la presencia de bacterias se evidenció únicamente en dos hemocultivos y en ninguna de las muestras se detectó la presencia de bacterias con el método de RT-PCR. Conclusiones. La PCR cuantitativa no mostró mayor eficacia que el BacT-ALERT 3D ® en la detección de la bacteriemia de origen oral.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Bacteremia / Colorimetry / Culture Techniques / Diabetes Mellitus, Type 2 / Chronic Periodontitis / Real-Time Polymerase Chain Reaction Type of study: Diagnostic study / Etiology study Limits: Aged / Female / Humans / Male Language: English Journal: Biomédica (Bogotá) Journal subject: Medicine Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bacteremia / Colorimetry / Culture Techniques / Diabetes Mellitus, Type 2 / Chronic Periodontitis / Real-Time Polymerase Chain Reaction Type of study: Diagnostic study / Etiology study Limits: Aged / Female / Humans / Male Language: English Journal: Biomédica (Bogotá) Journal subject: Medicine Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR