Hypothermia treatment ameliorated cyclin-dependent kinase 5-mediated inflammation in ischemic stroke and improved outcomes in ischemic stroke patients
Clinics
;
74: e938, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1039559
ABSTRACT
OBJECTIVES:
The inflammatory response is a key mechanism of neuronal damage and loss during acute ischemic stroke. Hypothermia has shown promise as a treatment for ischemic stroke. In this study, we investigated the molecular signaling pathways in ischemic stroke after hypothermia treatment.METHODS:
Cyclin-dependent kinase 5 (CDK5) was overexpressed or silenced in cultured cells. Nuclear transcription factor-κB (NF-κB) activity was assessed by measurement of the luciferase reporter gene. An ischemic stroke model was established in Sprague-Dawley (SD) rats using the suture-occluded method. Animals were assigned to three groups sham operation control, ischemic stroke, and ischemic stroke + hypothermia treatment groups. Interleukin 1β (IL-1β) levels in the culture supernatant and blood samples were assessed by ELISA. Protein expression was measured by Western blotting.RESULTS:
In HEK293 cells and primary cortical neuronal cultures exposed to hypothermia, CDK5 overexpression was associated with increased IL-1β, caspase 1, and NF-κB levels. In both a murine model of stroke and in patients, increased IL-1β levels were observed after stroke, and hypothermia treatment was associated with lower IL-1β levels. Furthermore, hypothermia-treated patients showed significant improvement in neurophysiological functional outcome.CONCLUSIONS:
Overall, hypothermia offers clinical benefit, most likely through its effects on the inflammatory response.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Brain Ischemia
/
NF-kappa B
/
Cyclin-Dependent Kinase 5
/
Interleukin-1beta
/
Hypothermia, Induced
/
Inflammation
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2019
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
The First Affiliated Hospital of Harbin Medical University/CN
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