Your browser doesn't support javascript.
loading
Effects of 1, 25-dihydroxyvitamin D3 on experimental periodontitis and AhR/NF-κB/NLRP3 inflammasome pathway in a mouse model
Li, Hao; Zhong, Xinghua; Li, Wei; Wang, Qi.
  • Li, Hao; Guangxi Medical University. The Affiliated Hospital of Stomatology. Department of Prosthodontics. CN
  • Zhong, Xinghua; Guangxi Medical University. The Affiliated Hospital of Stomatology. Department of Prosthodontics. CN
  • Li, Wei; Sichuan University. West China Hospital of Stomatology. State Key Laboratory of Oral Diseases. CN
  • Wang, Qi; Sichuan University. West China Hospital of Stomatology. State Key Laboratory of Oral Diseases. CN
J. appl. oral sci ; 27: e20180713, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1040234
ABSTRACT
Abstract Vitamin D has been known to have important regulatory functions in inflammation and immune response and shows inhibitory effects on experimental periodontitis in animal models. However, the potential mechanism has yet to be clarified. Recent studies have highlighted Aryl hydrocarbon receptor (AhR) and its downstream signaling as a crucial regulator of immune homeostasis and inflammatory regulation.

Objective:

This study aimed to clarify the effect of 1,25-dihydroxyvitamin D3 (VD3) on experimental periodontitis and AhR/nuclear factor-κB (NF-κB)/NLR pyrin domain-containing 3 (NLRP3) inflammasome pathway in the gingival epithelium in a murine model.

Methodology:

We induced periodontitis in male C57BL/6 wild-type mice by oral inoculation of Porphyromonas gingivalis (P. gingivalis), and subsequently gave intraperitoneal VD3 injection to the mice every other day for 8 weeks. Afterwards, we examined the alveolar bone using scanning electron microscopy (SEM) and detected the gingival epithelial protein using western blot analysis and immunohistochemical staining.

Results:

SEM images demonstrated that alveolar bone loss was reduced in the periodontitis mouse model after VD3 supplementation. Western blot analyses and immunohistochemical staining of the gingival epithelium showed that the expression of vitamin D receptor, AhR and its downstream cytochrome P450 1A1 were enhanced upon VD3 application. Additionally, VD3 decreased NF-κB p65 phosphorylation, and NLRP3, apoptosis-associated speck-like protein, caspase-1, interleukin-1β (IL-1β) and IL-6 protein expression.

Conclusions:

These results implicate the alleviation of periodontitis and the alteration of AhR/NF-κB/NLRP3 inflammasome pathway by VD3 in the mouse model. The attenuation of this periodontal disease may correlate with the regulation of AhR/NF-κB/NLRP3 inflammasome pathway by VD3.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Calcitriol / NF-kappa B / Bone Density Conservation Agents / NLR Family, Pyrin Domain-Containing 3 Protein Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Guangxi Medical University/CN / Sichuan University/CN

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Calcitriol / NF-kappa B / Bone Density Conservation Agents / NLR Family, Pyrin Domain-Containing 3 Protein Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Guangxi Medical University/CN / Sichuan University/CN