Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
Mem. Inst. Oswaldo Cruz
;
112(11): 785-789, Nov. 2017. graf
Article
in English
| LILACS
| ID: biblio-1040564
ABSTRACT
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.
Full text:
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Index:
LILACS (Americas)
Main subject:
Cytidine Deaminase
/
Deoxycytidine
/
Macrophages
/
Mycobacterium tuberculosis
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Pontifícia Universidade Católica do Rio Grande do Sul/BR
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