Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
Mem. Inst. Oswaldo Cruz
;
112(2): 155-159, Feb. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-1040566
ABSTRACT
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. FINDINGS Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. MAIN CONCLUSION Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
Full text:
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Index:
LILACS (Americas)
Main subject:
Leishmania braziliensis
/
Amphotericin B
/
Green Fluorescent Proteins
/
Drug Evaluation, Preclinical
/
Antiprotozoal Agents
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Viçosa/BR
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