Detection of occult hepatitis B in serum and oral fluid samples
Mem. Inst. Oswaldo Cruz
;
113(1): 62-65, Jan. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-1040579
ABSTRACT
In occult hepatitis B infection (OBI), hepatitis B virus DNA (HBV DNA) can be detected in serum samples; however, oral fluid collection for detection of HBV DNA has not yet been explored, despite the availability of collection devices. Serum and oral fluid samples from 45 hepatitis B core antibody (anti-HBc)-positive patients were collected for the amplification of the HBV polymerase gene. HBV DNA was detected in five serum and four oral fluid samples (the detection limit for oral fluid was 1.656 log IU/mL in paired serum). In conclusion, simple methodologies of sample collection and in-house polymerase chain reaction (PCR) allowed detection of HBV DNA, and these could be used to improve the diagnosis of OBI, especially in locations with limited resources.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Saliva
/
DNA, Viral
/
Hepatitis B
/
Hepatitis B Antibodies
/
Hepatitis B Surface Antigens
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz-Fiocruz/BR
/
Hospital Geral de Nova Iguaçu/BR
/
Universidade Federal do Estado do Rio de Janeiro/BR
/
Universidade Federal do Rio de Janeiro/BR
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