Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types
Mem. Inst. Oswaldo Cruz
;
113(10): e180340, 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-1040583
ABSTRACT
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Fungal
/
DNA Primers
/
Histoplasma
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2018
Type:
Article
Affiliation country:
Brazil
/
Mexico
Institution/Affiliation country:
Fundação Oswaldo Cruz-Fiocruz/BR
/
Universidad Nacional Autónoma de México/MX
/
Universidade Federal Fluminense/BR
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