Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis
Mem. Inst. Oswaldo Cruz
;
113(3): 197-201, Mar. 2018. graf
Article
in English
| LILACS
| ID: biblio-1040592
ABSTRACT
Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensitivity. The present study describes the recombinant protein rLc36, expressed by Leishmania infantum, as potential antigen for more sensitive and specific diagnosis of CVL based on an immunoenzymatic assay. The concentration of 1.0 μg/mL of rLc36 enabled differentiation of positive and negative sera and showed a sensitivity of 85% and specificity of 71% (with 95% confidence), with an accuracy of 76%.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Protozoan Proteins
/
Leishmania infantum
/
Dog Diseases
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista/BR
/
Universidade Federal de São Carlos/BR
/
Universidade de São Paulo/BR
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