Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
Mem. Inst. Oswaldo Cruz
;
113(8): e180086, 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-1040598
ABSTRACT
Dynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs). A putative PAT (TcPAT1) has been previously identified in Trypanosoma cruzi, the etiological agent of Chagas disease. Here we analyse other 14 putative TcPATs and 2 PPTs in the parasite genome. T. cruzi cell lines expressing TcPATs and TcPPTs plus a FLAG tag at the C terminus were produced for most enzymes, with positive detection by indirect immunofluorescence. Overexpressed TcPATs were mostly found as single spots at the parasite anterior end, while the TcPPTs were dispersed throughout the parasite body.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Palmitates
/
Trypanosoma cruzi
/
Protozoan Proteins
/
Protein S
/
Lipoylation
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz-Fiocruz/BR
/
Universidade Estadual de Ponta Grossa/BR
Similar
MEDLINE
...
LILACS
LIS