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Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin
de Souza, Renata Perotto; Favero, Vivian; Pascoal, Vanessa Fey; Lindholz, Catieli; Bittencourt, Hélio Radke; Graeff-Teixeira, Carlos.
  • de Souza, Renata Perotto; Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Porto Alegre. BR
  • Favero, Vivian; Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Porto Alegre. BR
  • Pascoal, Vanessa Fey; Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Porto Alegre. BR
  • Lindholz, Catieli; Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Porto Alegre. BR
  • Bittencourt, Hélio Radke; Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Porto Alegre. BR
  • Graeff-Teixeira, Carlos; Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Porto Alegre. BR
Mem. Inst. Oswaldo Cruz ; 114: e180529, 2019. tab, graf
Article in English | LILACS | ID: biblio-1040608
ABSTRACT
Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments.
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Full text: Available Index: LILACS (Americas) Main subject: Ovum / Parasite Egg Count / Schistosoma mansoni / Feces / Indicators and Reagents / Ninhydrin Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pontifícia Universidade Católica do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Ovum / Parasite Egg Count / Schistosoma mansoni / Feces / Indicators and Reagents / Ninhydrin Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pontifícia Universidade Católica do Rio Grande do Sul/BR