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Chronic granulomatous inflammation in teleost fish Piaractus mesopotamicus: histopathology model study / Inflamación crónica granulomatosa en el pez teleósteo Piaractus mesopotamicus: modelo de estudio histopatológico
Manrique, Wilson G; Figueiredo, Mayra AP; Belo, Marco AA; Martins, Maurício L; Moraes, Flávio R.
  • Manrique, Wilson G; São Paulo State University - UNESP. Department of Veterinary Pathology. Jaboticabal. BR
  • Figueiredo, Mayra AP; University of São Paulo. Department of Clinical Analysis. Toxicology and Food Science. Ribeirão Preto. BR
  • Belo, Marco AA; Brazil University. Descalvado Campus. Department of Veterinary Pathology. São Paulo. BR
  • Martins, Maurício L; Federal University of Santa Catarina - UFSC. Aquaculture Department - Aquatic Organisms Health Laboratory (AQUOS). Florianópolis. BR
  • Moraes, Flávio R; São Paulo State University - UNESP. Department of Veterinary Pathology. Jaboticabal. BR
Rev. MVZ Córdoba ; 22(1): 5738-5746, Jan.-Apr. 2017. graf
Article in English | LILACS | ID: biblio-1041179
Responsible library: CO140
ABSTRACT
ABSTRACT Objective. This study evaluated the cell kinetic and formation of granuloma during chronic inflammation induced by Bacillus Calmette-Guérin (BCG) in the skeletal muscle of Piaractus mesopotamicus, as a histopathology model to study innate immunity. Materials and methods. Sixty fish were divided in two groups BCG-inoculated and non-inoculated fish and the inflammatory response analyzed 3, 7, 14, 21 and 33 days post-inoculation (DPI) by histopathology after hematoxylin-eosin and Ziehl-Neelsen staining. Results. 3 DPI of BCG showed a diffuse inflammatory reaction mostly composed by mononuclear cells. The inflammation continued diffuse 7 DPI initiating the cellular organization surrounding the inoculum and have continued at 14 DPI with discrete presence of epithelioid-like type cells with acidophilic cytoplasm and floppy chromatin. Higher cellular organization (21 DPI) surrounding the granuloma with intense peripheral mononuclear inflammatory infiltrate and nevertheless, an increase in the number of fibroblasts and macrophage-like cells was observed. The inflammatory process became less diffuse 33 DPI with formation of small amount of granuloma surrounded by the same type of reaction found in bigger granuloma. Both the young and old granuloma presented typical characteristic around the inoculum composed by a layer of epithelioid-like type cells, besides macrophages, some lymphocytes and abundant fibroblasts. Conclusions. This study showed the feasibility in the use of pacus to study chronic granulomatous inflammatory response induced by BCG, characterized by changes in the kinetics of inflammatory cells in skeletal muscle classifying as immune-epithelioid type, similar to granulomatous inflammation caused by M. marinum in teleost fish.
RESUMEN
RESUMEN Objetivo. Este estudio evaluó la cinética celular y la formación de granuloma durante la inflamación crónica inducida por el Bacilo Calmette-Guérin (BCG) en el músculo esquelético de Piaractus mesopotamicus, como modelo histopatológico para estudiar la inmunidad innata. Materiales y métodos. Sesenta peces fueron divididos en dos grupos peces inoculados con BCG y no inoculados y la respuesta inflamatoria analizada en 3, 7, 14, 21 y 33 días post-inóculo (DPI) por medio del análisis histopatológico y tinciones de hematoxilina-eosina y Ziehl-Neelsen. Resultados. 3 DPI de BCG se observó reacción inflamatoria difusa principalmente formada por infiltrado celular mononuclear. Al 7° DPI la inflamación continuaba difusa con inicio de organización celular alrededor del inoculo, que se observó hasta el 14° DPI con discreta presencia de células de tipo epiteliodes con citoplasma acidófilo y cromatina laxa. Para el 21° DPI se observó alta organización celular alrededor del granuloma con intenso infiltrado mononuclear periférico e incremento en el número de fibroblastos y macrófagos. El proceso inflamatorio se tornó menos difuso a los 33 DPI con formación de pequeños granulomas contenidos dentro de uno más grande. Los granulomas formados más rápidamente así como los formados tardíamente, presentaron características típicas alrededor del inóculo compuesta por una camada de células tipo epitelioides, macrófagos, linfocitos y fibroblastos. Conclusiones. Este estudio mostró la viabilidad del uso del P. mesopotamicus para estudiar la respuesta inflamatoria crónica granulomatosa inducida con BCG, caracterizado por la evolución de la cinética de células inflamatorias en el músculo esquelético clasificándolo como de tipo inmune-epitelioide, similar a la inflamación granulomatosa causada por M. marinum en peces teleósteos.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Fishes / Granuloma / Macrophages / Mycobacterium Limits: Animals Language: English Journal: Rev. MVZ Córdoba Journal subject: Veterinary Medicine Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Brazil University/BR / Federal University of Santa Catarina - UFSC/BR / São Paulo State University - UNESP/BR / University of São Paulo/BR

Full text: Available Index: LILACS (Americas) Main subject: Fishes / Granuloma / Macrophages / Mycobacterium Limits: Animals Language: English Journal: Rev. MVZ Córdoba Journal subject: Veterinary Medicine Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Brazil University/BR / Federal University of Santa Catarina - UFSC/BR / São Paulo State University - UNESP/BR / University of São Paulo/BR