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A duplex endpoint PCR assay for rapid detection and differentiation of leptospira strains
Benacer, Douadi; Zain, Siti Nursheena Mohd; Lewis, John W; Khalid, Mohd Khairul Nizam Mohd; Thong, Kwai Lin.
  • Benacer, Douadi; University of Malaya. Faculty of Science. Institute of Biological Sciences. Kuala Lumpur. MY
  • Zain, Siti Nursheena Mohd; University of Malaya. Faculty of Science. Institute of Biological Sciences. Kuala Lumpur. MY
  • Lewis, John W; University of London. Royal Holloway. School of Biological Sciences. Egham. GB
  • Khalid, Mohd Khairul Nizam Mohd; Ministry of Health. Institute for Medical Research. Bacteriology Unit. Kuala Lumpur. MY
  • Thong, Kwai Lin; University of Malaya. Faculty of Science. Institute of Biological Sciences. Kuala Lumpur. MY
Rev. Soc. Bras. Med. Trop ; 50(2): 239-242, Mar.-Apr. 2017. tab, graf
Article in English | LILACS | ID: biblio-1041406
ABSTRACT
Abstract

INTRODUCTION:

This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.

METHODS:

Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.

RESULTS:

The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.

CONCLUSIONS:

Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
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Full text: Available Index: LILACS (Americas) Main subject: DNA, Bacterial / Polymerase Chain Reaction / DNA Primers / Leptospira Type of study: Diagnostic study / Screening study Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2017 Type: Article Affiliation country: Malaysia / United kingdom Institution/Affiliation country: Ministry of Health/MY / University of London/GB / University of Malaya/MY

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Full text: Available Index: LILACS (Americas) Main subject: DNA, Bacterial / Polymerase Chain Reaction / DNA Primers / Leptospira Type of study: Diagnostic study / Screening study Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2017 Type: Article Affiliation country: Malaysia / United kingdom Institution/Affiliation country: Ministry of Health/MY / University of London/GB / University of Malaya/MY