Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Rev. Soc. Bras. Med. Trop
;
51(1): 66-70, Jan.-Feb. 2018. graf
Article
in English
| LILACS
| ID: biblio-1041442
ABSTRACT
Abstract INTRODUCTION:
Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins.METHODS:
HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs.RESULTS:
The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs.CONCLUSIONS:
HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Virus Replication
/
Yellow fever virus
/
Virus Assembly
/
Vaccines, Virus-Like Particle
Limits:
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Fundação Oswaldo Cruz/BR
/
Universidade Federal do Pará/BR
/
University of Georgia/US
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