Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
Rev. Soc. Bras. Med. Trop
;
51(2): 237-239, Mar.-Apr. 2018. tab
Article
in English
| LILACS
| ID: biblio-1041455
ABSTRACT
Abstract INTRODUCTION:
Microscopic identification of active pulmonary tuberculosis (PTB) from direct smears of sputum (DS) is widely used for detection, but has limited sensitivity. Here, we assessed the yield of acid-fast bacilli (AFB) detection in processed sputum smears (PSS).METHODS:
Sputum samples were simultaneously analyzed by direct sputum smearing and after chemical treatment and spontaneous sedimentation.RESULTS:
Of the 1,719 samples analyzed, 16.4% were positive for AFB in conventional DS and 21.4% in PSS, corresponding to a 30% increase in detection.CONCLUSIONS:
Increased sensitivity from analyzing PSS and better safety protocols will contribute to improved detection and control of the disease.
Full text:
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Index:
LILACS (Americas)
Main subject:
Specimen Handling
/
Sputum
/
Tuberculosis, Pulmonary
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
/
Practice guideline
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz/BR
/
Laboratório Municipal de Saúde Pública/BR
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