Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
Rev. Soc. Bras. Med. Trop
;
51(3): 387-389, Apr.-June 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-1041461
ABSTRACT
Abstract INTRODUCTION:
Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated.METHODS:
We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA).RESULTS:
The conidia concentration required for an EIA-positive result was 4.8 × 103 (median).CONCLUSIONS:
This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Aspergillus fumigatus
/
Spores, Fungal
/
Enzyme-Linked Immunosorbent Assay
/
False Positive Reactions
/
Mannans
Limits:
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Pelotas/BR
/
Universidade Federal do Rio Grande/BR
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