Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR
Rev. Soc. Bras. Med. Trop
;
51(4): 528-532, July-Aug. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-1041475
ABSTRACT
Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. RESULTS:
The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations.CONCLUSIONS:
Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Staphylococcus aureus
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Bacteriological Techniques
Type of study:
Diagnostic study
/
Practice guideline
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Minas Gerais/BR
/
Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR
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