Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
Rev. Soc. Bras. Med. Trop
;
51(5): 665-669, Sept.-Oct. 2018. graf
Article
in English
| LILACS
| ID: biblio-1041484
ABSTRACT
Abstract INTRODUCTION:
Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation.METHODS:
We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays.RESULTS:
Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results.CONCLUSIONS:
The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
Full text:
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Index:
LILACS (Americas)
Main subject:
Urea
/
Immunoglobulin G
/
Antibodies, Protozoan
/
Leishmaniasis
/
Chagas Disease
/
Cross Reactions
/
Antibody Affinity
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Butantan/BR
/
Secretaria de Estado da Saúde de São Paulo/BR
/
Universidade de São Paulo/BR
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