PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil
Rev. Soc. Bras. Med. Trop
;
51(6): 843-847, Nov.-Dec. 2018. tab
Article
in English
| LILACS
| ID: biblio-1041493
ABSTRACT
Abstract INTRODUCTION Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. METHODS PCR and parasitological tests were conducted. RESULTS PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples. CONCLUSIONS Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.
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Index:
LILACS (Americas)
Main subject:
Ehrlichiosis
/
Dog Diseases
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Controle de Zoonozes de Campo Grande/BR
/
Fundação Oswaldo Cruz/BR
/
Universidade Federal da Grande Dourados/BR
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