IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Rev. Soc. Bras. Med. Trop
;
52: e20180139, 2019. graf
Article
in English
| LILACS
| ID: biblio-1041506
ABSTRACT
Abstract INTRODUCTION:
High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.METHODS:
Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.RESULTS:
Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.CONCLUSIONS:
Potato apyrase and SmATPDases have IgE-binding epitopes.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Apyrase
/
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Solanum tuberosum
/
Immunoglobulin E
/
Antibodies, Helminth
/
Epitopes
Limits:
Animals
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2019
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz/BR
/
Universidade Federal de Juiz de Fora/BR
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