Rapid detection of sepsis using cesda: the caenorhabditis elegans sepsis detection assay
Rev. Soc. Bras. Med. Trop
;
52: e20180300, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1041561
ABSTRACT
Abstract INTRODUCTION The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS CESDA could be further explored for sepsis diagnosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biomarkers
/
Chemotaxis
/
Caenorhabditis elegans
/
Sepsis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2019
Type:
Article
Affiliation country:
Malaysia
Institution/Affiliation country:
Universiti Kebangsaan Malaysia/MY
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