Comparison of methodologies for detecting Trypanosoma cruzi parasites by microscopic observation of microhematocrit capillary tubes
Rev. Soc. Bras. Med. Trop
;
52: e20180505, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1041570
ABSTRACT
Abstract INTRODUCTION:
The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood.METHODS:
The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity.RESULTS:
The rotating method was easier, faster, and more sensitive than the others with 100% specificity.CONCLUSIONS:
The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.
Full text:
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Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Centrifugation
/
Chagas Disease
/
Parasitemia
/
Capillary Tubing
/
Hematocrit
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2019
Type:
Article
Affiliation country:
Belgium
/
Mexico
Institution/Affiliation country:
Universidad Autónoma de Yucatán/MX
/
Université Libre de Bruxelles/BE
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