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Diversity and abundance of denitrifiers during cow manure composting / Diversidad y abundancia de desnitrificadores durante el compostaje del estiércol de vaca
Meng, Qingxin; Xu, Xiuhong; Zhang, Wenhao; Cheng, Lijun; Men, Mengqi; Xu, Benshu; Deng, Liting; Sun, Xuewei.
  • Meng, Qingxin; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Xu, Xiuhong; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Zhang, Wenhao; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Cheng, Lijun; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Men, Mengqi; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Xu, Benshu; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Deng, Liting; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
  • Sun, Xuewei; Northeast Agricultural University. College of Resources and Environment. Harbin. CN
Rev. argent. microbiol ; 51(3): 191-200, set. 2019. ilus, graf, tab
Article in English | LILACS | ID: biblio-1041824
ABSTRACT
Diversity and abundance of the denitrifying genes nirK, nirS and nosZ were investigated in cow manure compost using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time quantitative PCR (qPCR), respectively. These three genes were detected in all the stages of the composting process. Phylogenetic analysis showed that the nirK gene was closely related to Rhizobiales, Burkholderiales, the nirS gene was closely related to Pseudomonadales and Burkholderiales, and the nosZ gene was closely related to Rhodospirillales, Rhizobiales, Pseudomonadales, and Alteromonadales. qPCR results showed that the abundance of these three genes (nirK, nirS and nosZ) reached the peak value in the late thermophilic stage of composting and abundance of the nirK gene was higher than that of the nosZ gene and the nirS gene. Redundancy analysis (RDA) showed that the diversity of the nirK and nirS genes was significantly correlated with ammonium (p < 0.05), the diversity of the nosZ gene was significantly correlated with pH (p < 0.05) and the abundance of the nirK nirS and nosZ genes was significantly correlated with temperature (p< 0.05).
RESUMEN
La diversidad y la abundancia de los genes desnitrificadores nirK, nirS, nosZ en el compost de estiércol de vaca se investigaron por medio de la reacción en cadena de la polimerasa seguida de electroforesis en gel con gradiente de desnaturalización (PCR-DGGE) y por PCR cuantitativa (qPCR) en tiempo real, respectivamente. Estos 3 genes fueron detectados durante todas las fases del compostaje. El análisis filogenético mostró estrecha relación del gen nirK con Rhizobiales y Burkholderiales, del gen nirS con Pseudomonadales y Burkholderiales y del gen nosZ con Rhodospirillales, Rhizobiales, Pseudomonadales y Alteromonadales. Los resultados de la qPCR mostraron que la abundancia de los genes nirK, nirSy nosZ alcanzó el valor máximo en la fase termofílica tardía del compostaje, y que la abundancia del gen nirK era más elevada que los de los genes nosZ y nirS. El análisis de redundancia (RDA) mostró que la diversidad de los genes nirK y nirS estaba significativamente correlacionada con la concentración de amonio (p<0,05), mientras que la del gen nosZ estaba significativamente correlacionada con el pH (p<0,05). También mostró que la abundancia de los genes nirK, nirS y nosZ estaba significativamente correlacionada con la temperatura (p<0,05).
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Soil Microbiology / Composting / Denitrification / Genes, Bacterial Limits: Animals Language: English Journal: Rev. argent. microbiol Journal subject: Microbiology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Northeast Agricultural University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Soil Microbiology / Composting / Denitrification / Genes, Bacterial Limits: Animals Language: English Journal: Rev. argent. microbiol Journal subject: Microbiology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Northeast Agricultural University/CN