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Transcriptome analysis for the restrained stem development of the wheat mutant dms / Desenvolvimento e análise de transcriptoma de uma haste de trigo mutante dms
He, Ruishi; Zhu, Xinxin; Li, Qiaoyun; Jiang, Yumei; Yu, Dongyan; Sun, Yulong; Liang, Xiaolong; Ni, Yongjing; Niu, Jishan.
  • He, Ruishi; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Zhu, Xinxin; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Li, Qiaoyun; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Jiang, Yumei; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Yu, Dongyan; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Sun, Yulong; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Liang, Xiaolong; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Ni, Yongjing; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
  • Niu, Jishan; Henan Agricultural University. Centre of Engineering and Technological Research for Wheat. Zhengzhou. CN
Ciênc. rural (Online) ; 47(12): e20170241, Dec. 2017. tab, graf
Article in English | LILACS | ID: biblio-1044938
ABSTRACT
ABSTRACT Wheat (Triticum aestivum L.) stem development significantly affects grain yield. The dwarf plants (D) of wheat mutant dms was less than 30cm. Here, we were to explore the molecular basis for the restrained stem development of the dwarf plants. The results were reached by compare the young spikes and stems transcriptomes of the tall (T) and D plants of mutant dms. We identified 663 genes highly expressed in stem tips. We identified 997 differentially expressed genes (DEGs) in stem tips between T and D, 403 DEGs were significantly related with stem development. Most biological processes in stem tips on dwarf plants were significantly suppressed, such as phytohormone signaling etc. The sequencing analysis results were confirmed by quantitatively analysis the expression profiles of fourteen key DEGs via real-time QRT-PCR. We identified a group genes related to wheat stem development, identified a group DEGs related to the restrained stem development of D plants of dms. The suppressed phytohormone signaling, carbohydrate transport and metabolism were the major causal factors leading to dwarf plants of D. Our dataset provides a useful resource for investigating wheat stem development.
RESUMO
RESUMO O desenvolvimento da haste do trigo (Triticum aestivum L.) afeta significativamente o rendimento dos grãos. A partir disso, empregou-se a base molecular para o desenvolvimento de uma haste de variedade de trigo. Os resultados foram alcançados ao conferir as hastes de um mutante de trigo DMS. Identificou-se 663 genes altamente expressos na haste; 997 genes (DEGs) diferentemente expressos em hastes entre T e D e; 403 DEGs foram significativamente diferentes. A maioria dos processos biológicos de caule tipo não plantas foram significativamente suprimidas, com o fito hormônio de sinalização. Os resultados da análise de sequencia foram confirmados pela expressão quantitativa de perfis de catorze chave DEGs através de qRT-PCR em tempo real. Nota-se um grupo de genes relacionados com a haste de trigo de desenvolvimento. Identificou-se um grupo DEGs relacionados com o desenvolvimento de um tronco D plantas de DMS. O fito hormônio de sinalização, transporte e metabolismo de hidratos de carbono foram os principais fatores suscetíveis das plantas anão de D. Nosso conjunto de dados fornece um recurso útil para investigar o desenvolvimento do caule de trigo.


Full text: Available Index: LILACS (Americas) Type of study: Prognostic study Language: English Journal: Ciênc. rural (Online) Journal subject: Sa£de Ambiental Year: 2017 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Henan Agricultural University/CN

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Full text: Available Index: LILACS (Americas) Type of study: Prognostic study Language: English Journal: Ciênc. rural (Online) Journal subject: Sa£de Ambiental Year: 2017 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Henan Agricultural University/CN