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Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
Li, Shanshan; Qin, Kun; Li, Huaying; Guo, Jin; Li, Dejin; Liu, Fang; Tan, Zhilei; Yan, Wei; Qu, Shuling; Zhao, Huabing.
  • Li, Shanshan; Xi'an Jiaotong University. Department of Environmental Science & Engineering. Xi'an. CN
  • Qin, Kun; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
  • Li, Huaying; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
  • Guo, Jin; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
  • Li, Dejin; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
  • Liu, Fang; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
  • Tan, Zhilei; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
  • Yan, Wei; Xi'an Jiaotong University. Department of Environmental Science & Engineering. Xi'an. CN
  • Qu, Shuling; Tuanbowa Development Company of Dagang Oilfield Corporation. Tianjin. CN
  • Zhao, Huabing; Logistics University of Chinese People's Armed Police Forces. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards. Tianjin. CN
Electron. j. biotechnol ; 34: 83-90, july. 2018. tab, ilus, graf
Article in English | LILACS | ID: biblio-1047375
ABSTRACT

Background:

Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes.

Results:

In this study, four novel C12O genes, designated catA, catAI, catAII and catAIII, in the naphthalene-degrading strain Pseudomonas putida ND6, were cloned and characterised. Phylogenetic analysis of their deduced amino acid sequences revealed that the four C12O isozymes each formed independent subtrees, together with homologues from other organisms. All four enzymes exhibited maximum activity at pH 7.4 and higher activity in alkaline than in acidic conditions. Furthermore, CatA, CatAI and CatAIII were maximally active at a temperature of 45°C, whereas a higher optimum temperature was observed for CatAII at a temperature of 50°C. CatAI exhibited superior temperature stability compared with the other three C12O isozymes, and kinetic analysis indicated similar enzyme activities for CatA, CatAI and CatAII, whereas that of CatAIII was lower. Significantly, among metal ions tested, only Cu2+ substantially inhibited the activity of these C12O isozymes, thus indicating that they have potential to facilitate bioremediation in environments polluted with aromatics in the presence of metals. Moreover, gene expression analysis at the mRNA level and determination of enzyme activity clearly indicated that the redundancy of the catA genes has increased the levels of C12O.

Conclusion:

The results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas putida / Catechol 1,2-Dioxygenase Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Logistics University of Chinese People's Armed Police Forces/CN / Tuanbowa Development Company of Dagang Oilfield Corporation/CN / Xi'an Jiaotong University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas putida / Catechol 1,2-Dioxygenase Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Logistics University of Chinese People's Armed Police Forces/CN / Tuanbowa Development Company of Dagang Oilfield Corporation/CN / Xi'an Jiaotong University/CN